The company has tightened its belt at a time of rising staff wages and repairs.
Spokesman Eddie Gershon said the pub chain understands that "customers and staff will be disappointed" but they had to make the move.
The venues will be marketed by commercial property specialists CBRE and Savills.
Mr Gershon said: "On occasion, Wetherspoon does put some of its pubs up for sale.
"This is a commercial decision. We understand that customers and staff will be disappointed with it.
"The pubs will continue to operate as Wetherspoon outlets until they are sold."
The 32 pubs are:
Barnsley – Silkstone Inn
Beaconsfield – Hope & Champion
Bexleyheath – Wrong ‘Un
Bournemouth – Christopher Creeke
Cheltenham – Bank House
Durham – Water House
Halifax – Percy Shaw
Hanham – Jolly Sailor
Harrow – Moon on the Hill
Hove – Cliftonville Inn
London Battersea – Asparagus
London East Ham – Miller's Well
London Eltham – Bankers Draft
London Forest Gate – Hudson Bay
London Forest Hill – Capitol
London Hornsey – Toll Gate
London Holborn – Penderel's Oak
London Islington – Angel
London Palmers Green – Alfred Herring
Loughborough – Moon & Bell
Loughton – Last Post
Mansfield – Widow Frost
Middlesborough – Resolution
Purley – Foxley Hatch
Redditch – Rising Sun
Sevenoaks – Sennockian
Southampton – Admiral Sir Lucius Curtis
Stafford – Butler's Bell
Watford – Colombia Press
West Bromwich – Billiard Hall
Willenhall – Malthouse
Wirral – John Masefield
CBRE senior director Toby Hall said: "The excellent mix of locations in this portfolio is rarely seen in the market.
"With more than half of the portfolio located in London and the south-east and other strong locations in the south-west, Midlands and the north of England we believe the pubs represent an excellent opportunity for existing pub operators and new entrants."
JD Wetherspoon
Tim Ireland
Founder and Chairman of JD Wetherspoon, Tim Martin
PA