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Camilla, Duchess of Cornwall, has tested positive for Covid-19 as concerns grow for the health of the Queen.
A Clarence House spokesman said: "Her Royal Highness The Duchess of Cornwall has tested positive for Covid-19 and is self-isolating.
Camilla, 74, caught the virus for the first time just days after the Prince of Wales contracted the illness for a second time.
"We continue to follow government guidelines."
Camilla is triple vaccinated, sources said.
The duchess carried out a string of engagements last Thursday – the day Charles tested positive.
Camilla described herself as “luckily” negative the same day, saying of her testing regime during a visit to Thames Valley Partnership in Buckinghamshire: “I’ve taken it so many times”.
Prince Charles' positive test result last week generated concerns that Queen Elizabeth had also been exposed.
The Queen did not display any symptoms following Charles' positive result, but Buckingham Palace refused to confirm whether she had tested positive or negative, citing medical privacy.
The 95-year-old is believed to have spent time with Charles on Tuesday when her eldest son was carrying out an investiture on her behalf at her Windsor Castle home.
Several public figures expressed concern for the Queen's health online, including Piers Morgan who tweeted: "Prince Charles testing positive for covid just 2 days after seeing the Queen is filling me with deep unease."